Postoctoral position in the metabolic phenotype of tumor cells in defined conditions of the microenvironment – in the context of 13C- magnetic resonance spectroscopy

Posted: 
Saturday, 30 May 2015
Expires: 
Monday, 22 June 2015

Project description: The altered metabolism of tumors can be monitored with nuclear magnetic resonance (NMR) spectroscopy using NMR-active metabolites. With the implementation of hyperpolarized 13C-molecules such as 13C–pyruvate, it has become possible to measure the metabolic dynamics within a few seconds. The evaluation of the NMR-signals requires knowledge on the influence of the tumor microenvironment on the cellular uptake of metabolites, especially on how nutrient and oxygen deprivation as well as an acidic pH modulate the cellular uptake of 13C-pyruvate. For this, 3D in-vitro tumor cell structures (spheroids) maintained in various tumor-like growth conditions serve as model systems. Further biochemical investigations will focus on 1) the cellular uptake of 14C-pyruvate, 2) the activity of metabolic enzymes, and 3) the metabolic flux of 13C-pyruvate using mass spectroscopy (isotopologue profiling). This project will be done in close cooperation with the Department of Biochemistry and the Clinic for Nuclear Medicine (Klinikum rechts der Isar) at the TUM.

 

Requirements: The candidate will have a doctoral degree (PhD), a track record of a sound theoretical and practical background in biochemistry and cell biology, preferably in oncology. Experience in working with cells and biochemical assays as required above would be a major advantage. He/she should be dedicated, creative, and diligent in performing sophisticated experiments, and have good skills in communicating concepts and results in good English, including writing publications.
The postdoctoral position is funded for two years. 
Please see the website (http://www.imetum.tum.de/about-the-institute/?L=1) for a description of the institute and address any further queries to otto@tum.de.
The application, including a cover letter, full CV, a brief description of scientific achievements and interests (publications, meetings, awards, etc.), and contact information of 2-3 referees, should be sent to Dr. Angela Otto (otto@tum.de).

 

Qualifications: 
PhD
Salary: 
The salary will be according to the university tariff (TV-L).
Employers: 
Angela Otto
Job type: 
PostDoctoral position of two years
Location: 
Zentralinstitut für Medizintechnik, Technische Universität München
Contact: 
otto@tum.de Tel +49 89 289-10806 Fax +49 89 289-10805